Hughes was a resident of Sevastopol Station. When a lone Xenomorph was unleashed on the station by the crew of the Anesidora in 2137, he became one of the survivors desperately waiting for rescue. He was encountered by Amanda Ripley when she arrived on Sevastopol to recover the USCSS Nostromo's flight recorder, which had also been brought there by the Anesidora.
Hughes was killed by a Working Joe android when he attempted to reactivate the station's communications systems to call for help.
He was seen arguing with a Seegson Synthetic who was positioned to defend the elevator. He attempted to enter the elevator but was caught by the synthetic. An argument started after which eventually led them to fight leading to Hughes shooting at the Working Joe. Provoking it to throw Hughes against a wall and having his head brutally smashed in against the wall by the synthetic, killing him.